The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane

The AAA protein Msp1 mediates clearance of excess tail-anchored proteins from the peroxisomal membrane

Msp1 is a conserved AAA ATPase in budding yeast localized to mitochondria where it prevents accumulation of mistargeted tail-anchored (TA) proteins, including the peroxisomal TA protein Pex15. Msp1 also resides on peroxisomes but it remains unknown how native TA proteins on mitochondria and peroxisomes evade Msp1 surveillance. We used live-cell quantitative cell microscopy tools and drug-induci...

Title: The AAA Protein Msp1 Mediates Clearance of Excess Tail-Anchored Proteins from the Peroxisomal Membrane

The conserved AAA-ATPase Msp1 confers organelle specificity to tail-anchored proteins.

The accuracy of tail-anchored (TA) protein targeting to the endoplasmic reticulum (ER) depends on the Guided Entry of Tail-Anchored (Get) protein targeting machinery. The fate of TA proteins that become inappropriately inserted into other organelles, such as mitochondria, is unknown. Here, we identify Msp1, a conserved, membrane-anchored AAA-ATPase (ATPase associated with a variety of cellular ...

The GET Complex Mediates Insertion of Tail-Anchored Proteins into the ER Membrane

Tail-anchored (TA) proteins, defined by the presence of a single C-terminal transmembrane domain (TMD), play critical roles throughout the secretory pathway and in mitochondria, yet the machinery responsible for their proper membrane insertion remains poorly characterized. Here we show that Get3, the yeast homolog of the TA-interacting factor Asna1/Trc40, specifically recognizes TMDs of TA prot...

The novel tail-anchored membrane protein Mff controls mitochondrial and peroxisomal fission in mammalian cells.

Few components of the mitochondrial fission machinery are known, even though mitochondrial fission is a complex process of vital importance for cell growth and survival. Here, we describe a novel protein that controls mitochondrial fission. This protein was identified in a small interfering RNA (siRNA) screen using Drosophila cells. The human homologue of this protein was named Mitochondrial fi...